Direct analysis by UHPLC-MS/MS of 8 purified fractions from ethanolic extracts of Talipariti elatum's flowers in Martinica
Abstract
From ethanolic extracts of the flowers of Talipariti elatum (Sw.) eigth different samples were isolated, purified and analyzed by UHPLC-MS/MS to determine the chemical constituents that they content. Each sample was separated and reinjected to get the most possible information about the chemical compounds that they posses. After an exhaustive analysis 8 different chemical components were tentatively identified according to their MS and literature data. Two compounds until remain unknown.
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Introduction
Talipariti elatum Sw. (Fryxell) of the Malvaceae family is a tree native of Cuba and Jamaica. It is called « Majagua azul » in Cuba, « Mahot bleu » or « Mahot de montagne » in Martinique (US Dpt. of Ag., 2013). It produces beautiful orange to red flowers throughout the year (Figure 1). Domesticated then planted in almost all the islands and countries of the Caribbean basin, it is used for ornamental but also for medicinal purposes. Its flowers are widely used in the Cuban pharmacopoeia for coughs, asthma, catarrh, etc., (Roig, 1974; Acosta y Rodríguez, 2006) where two phytomedication"Imefasma" and"Flormaj" have already been manufactured and marketed in that country.
Despite widespread use in the Caribbean and undeniable pharmacological potential, there is very little information on its phytochemical constituents in the literature.The ARVARNAM and BIOSPHERES research groups with the University of Havana united in collaborative project therefore undertook to study this plant to characterize its secondary metabolites. The first analyzes by UPLC-DAD-ESI-MS/MS of the flower extract were therefore carried out and published in2017 where the structures of 12 chemical compounds were elucidatedafterisolation. The aim of this research was to analyze eight differentes samples to get information about the chemical components present in each one.
Conclusion
A simple and versatile analytical method, the “UPLC-DAD-ESI-MS / MS” was implemented to allow direct identification of the constituents inhydroalcoholic extract of the flower petals of Talipariti elatum (Sw.). The analysis was carried out by RP HPLC coupled to a DAD detector and to a tandem ion trap mass spectrometer in order to obtain a UV profile and a spectrum of fragmentations in negative mode making it possible to achieve provisional identification. This study was also accompanied by the analysis of the extraction precipitate and the first attempts to purify the majority constituents of the extract. Eights compounds have thus been tentatively identified, all of them reported for the first time in the ethanolic extracts of this part of the plant. Thus, it has been demonstrated that gossypetin-O-glucoside does not precipitate alone and that the use of UPLC-DAD for preparatory purposes is promising. We were able to isolate four major products including gossypetin-O-glucoside.